cells tlr4  (InvivoGen)

Journal: Frontiers in Toxicology

Article Title: Microplastics amplify the pro-inflammatory response to fungal mycelial fragments and spores in neutrophil-like cells

doi: 10.3389/ftox.2026.1718466

Figure Lengend Snippet: Bar plots of the fold changes following submerged exposure of reporter cells to test materials that include AFMHDPE (AFM (10 6 /mL) + HDPE (100 μg/mL)), AFM (10 6 /mL), AFSHDPE [AFS (10 6 /mL) + HDPE (100 μg/mL)], AFS (10 6 /mL) particles, H 2 O as the negative control, and positive controls [LPS (100 ng/mL) for TLR4 and LTA (100 ng/mL) for TLR2]. The blank control served as the background response without cells. The red stippled line indicates fold change level 2, which was considered the activation threshold. Data from two independent experiments are shown.

Article Snippet: In brief, TLR2 (InvivoGen #hkb-htlr2 Toulouse, France) and TLR4 (InvivoGen #hkb-htlr4) HEK-Blue reporter cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco #31966) supplemented with 10% heat-inactivated endotoxin-free fetal bovine serum (FBS; Biowest #S1860), 100 U/mL penicillin (Biowest #L0022 Nuaillé, France), 100 μg/mL streptomycin (Biowest #L0022), 100 μg/mL normocin (InvivoGen #ant-zn), and 1xHEK Selection-Blue (InvivoGen #hb-sel).

Techniques: Negative Control, Control, Activation Assay

Journal: iScience

Article Title: Fiber enrichment is not superior to dietary monitoring in MASLD: A dual-center, double-blind, placebo-controlled trial

doi: 10.1016/j.isci.2025.114019

Figure Lengend Snippet: Microbiome assessment and TLR2 and 4 ligands at the Run-in phase (A) PCoA based on phylo-RPCA distances. Locations are differentiated by markers and weeks by color. (B) Shannon entropy and Faith phylogenetic diversity boxplots by week, location, group, and sex. The line inside the box represents the median, while the whiskers represent the lowest and highest values (excluding outliers) and interquartile range (IQR). Independent samples were tested with the Kruskal-Wallis test, and dependent samples were tested with the Wilcoxon test. Significant p -values are indicated with asterisks (∗ - p < 0.05; ∗∗ - p < 0.01; ∗∗∗ - p < 0.001). (C) ANCOM-BC differential abundance test between locations. Colors indicate where a given taxon was found to be more abundant. Log fold changes plotted only for taxons with adjusted p -values less than 0.05. (D) Toll-like receptor 4 (TLR4) (Wilcoxon test, n = 34) and (E) TLR2 ligands (Wilcoxon test, n = 34) in the serum of participants at timepoint -3W and 0W. Single data points are presented in graph D and E.

Article Snippet: Human TLR4 Reporter HEK293 Cell Assay , Invivogen , Cat# hkb-htlr4, RRID:CVCL_IM82.

Techniques:

Journal: iScience

Article Title: Fiber enrichment is not superior to dietary monitoring in MASLD: A dual-center, double-blind, placebo-controlled trial

doi: 10.1016/j.isci.2025.114019

Figure Lengend Snippet: TLR2 and 4 ligands during dietary fiber and placebo intervention Linear mixed effects analyses were performed for intervention (weeks 0–12) and follow-up (weeks 12–20) stages with the placebo group as reference. (A) Toll-like receptor 4 (TLR4, oat bran n = 12 expect for 20W n = 11, spelt bran n = 11 expect for 20W n = 10, placebo n = 11) and (B) TLR2 ligands (oat bran n = 12 expect for 20W n = 11, spelt bran n = 11 expect for 20W n = 10, placebo n = 11) in serum of patients at timepoint 0W to 20W. Bands represent 95% confidence intervals. p -values and coefficients are plotted on the right side of the subplots.

Article Snippet: Human TLR4 Reporter HEK293 Cell Assay , Invivogen , Cat# hkb-htlr4, RRID:CVCL_IM82.

Techniques:

Journal: iScience

Article Title: Fiber enrichment is not superior to dietary monitoring in MASLD: A dual-center, double-blind, placebo-controlled trial

doi: 10.1016/j.isci.2025.114019

Figure Lengend Snippet: Microbiome composition of responder and non-responder subgroups and correlations with TLR2/TLR4 ligands (A) MaAsLin2 correlations between microbiota abundances and TLR2/TLR4 ligands in responder (R) and non-responder (N) subgroups within each group. (B–D) PcoA plots based on phylo-RPCA distances within each group. Responder (R) and non-responder subgroups are differentiated by markers and phases by color and marker size. In the right lower corner of each PCoA plot, response rate is plotted. Respond rate was calculated as the number of responders divided by the total number of participants within each group.

Article Snippet: Human TLR4 Reporter HEK293 Cell Assay , Invivogen , Cat# hkb-htlr4, RRID:CVCL_IM82.

Techniques: Marker

Journal: ACS Omega

Article Title: Modulating Receptor Activity, Immune Response, and Kinetic Solubility: The Impact of Linker Chemistry in Conjugated NOD2/TLR4 Agonists

doi: 10.1021/acsomega.5c05358

Figure Lengend Snippet: Chemical structures of the first reported conjugated NOD2/TLR4 agonists 1 and 2 .

Article Snippet: HEK-Blue hNOD2 cells (25,000 cells/well) and TLR4 cells (40,000 cells/well) were seeded in 96-well plates in 100 μL of HEK-Blue detection medium (Invivogen, San Diego, CA, USA) and treated with compounds (at 0.5 μM, 1 μM, 5 μM and 10 μM in the case of HEK-Blue hNOD2 cells (all conjugates) or with the corresponding vehicle (0.1% DMSO) and in the case of HEK-Blue hTLR4 cells at two different concentration ranges, 0.5 μM, 1 μM, 5 μM and 10 μM (conjugates 17–21 ) and 1 μM, 10 μM and 100 μM (conjugates 27–31 ) or the corresponding vehicle (0.1% DMSO).

Techniques:

Journal: ACS Omega

Article Title: Modulating Receptor Activity, Immune Response, and Kinetic Solubility: The Impact of Linker Chemistry in Conjugated NOD2/TLR4 Agonists

doi: 10.1021/acsomega.5c05358

Figure Lengend Snippet: Design of novel conjugated NOD2/TLR4 agonists.

Article Snippet: HEK-Blue hNOD2 cells (25,000 cells/well) and TLR4 cells (40,000 cells/well) were seeded in 96-well plates in 100 μL of HEK-Blue detection medium (Invivogen, San Diego, CA, USA) and treated with compounds (at 0.5 μM, 1 μM, 5 μM and 10 μM in the case of HEK-Blue hNOD2 cells (all conjugates) or with the corresponding vehicle (0.1% DMSO) and in the case of HEK-Blue hTLR4 cells at two different concentration ranges, 0.5 μM, 1 μM, 5 μM and 10 μM (conjugates 17–21 ) and 1 μM, 10 μM and 100 μM (conjugates 27–31 ) or the corresponding vehicle (0.1% DMSO).

Techniques:

Journal: ACS Omega

Article Title: Modulating Receptor Activity, Immune Response, and Kinetic Solubility: The Impact of Linker Chemistry in Conjugated NOD2/TLR4 Agonists

doi: 10.1021/acsomega.5c05358

Figure Lengend Snippet: Synthesis of Conjugated NOD2/TLR4 Agonists 17–21 Based on the TLR4 Agonist 4

Article Snippet: HEK-Blue hNOD2 cells (25,000 cells/well) and TLR4 cells (40,000 cells/well) were seeded in 96-well plates in 100 μL of HEK-Blue detection medium (Invivogen, San Diego, CA, USA) and treated with compounds (at 0.5 μM, 1 μM, 5 μM and 10 μM in the case of HEK-Blue hNOD2 cells (all conjugates) or with the corresponding vehicle (0.1% DMSO) and in the case of HEK-Blue hTLR4 cells at two different concentration ranges, 0.5 μM, 1 μM, 5 μM and 10 μM (conjugates 17–21 ) and 1 μM, 10 μM and 100 μM (conjugates 27–31 ) or the corresponding vehicle (0.1% DMSO).

Techniques:

Journal: ACS Omega

Article Title: Modulating Receptor Activity, Immune Response, and Kinetic Solubility: The Impact of Linker Chemistry in Conjugated NOD2/TLR4 Agonists

doi: 10.1021/acsomega.5c05358

Figure Lengend Snippet: Synthesis of Conjugated NOD2/TLR4 Agonists 27–31 Based on TLR4 Agonist 6

Article Snippet: HEK-Blue hNOD2 cells (25,000 cells/well) and TLR4 cells (40,000 cells/well) were seeded in 96-well plates in 100 μL of HEK-Blue detection medium (Invivogen, San Diego, CA, USA) and treated with compounds (at 0.5 μM, 1 μM, 5 μM and 10 μM in the case of HEK-Blue hNOD2 cells (all conjugates) or with the corresponding vehicle (0.1% DMSO) and in the case of HEK-Blue hTLR4 cells at two different concentration ranges, 0.5 μM, 1 μM, 5 μM and 10 μM (conjugates 17–21 ) and 1 μM, 10 μM and 100 μM (conjugates 27–31 ) or the corresponding vehicle (0.1% DMSO).

Techniques:

Journal: ACS Omega

Article Title: Modulating Receptor Activity, Immune Response, and Kinetic Solubility: The Impact of Linker Chemistry in Conjugated NOD2/TLR4 Agonists

doi: 10.1021/acsomega.5c05358

Figure Lengend Snippet: Concentration-dependent NF-κB transcriptional activities of conjugated NOD2/TLR4 agonists. SEAP activity was measured in (A) HEK-Blue hNOD2 cells after incubation for 18 h with MDP (positive control; 4 μM), and compounds (0.5–10 μM); and (B) HEK-Blue hTLR4 (B) cells after incubation for 18 h with LPS (positive control; 10 ng/mL) and compounds (0.5–10 μM or 1–100 μM concentration range as indicated). Data are shown as relative activities to the vehicle-treated control (0.1% DMSO) and are means ± SEM of three independent experiments.

Article Snippet: HEK-Blue hNOD2 cells (25,000 cells/well) and TLR4 cells (40,000 cells/well) were seeded in 96-well plates in 100 μL of HEK-Blue detection medium (Invivogen, San Diego, CA, USA) and treated with compounds (at 0.5 μM, 1 μM, 5 μM and 10 μM in the case of HEK-Blue hNOD2 cells (all conjugates) or with the corresponding vehicle (0.1% DMSO) and in the case of HEK-Blue hTLR4 cells at two different concentration ranges, 0.5 μM, 1 μM, 5 μM and 10 μM (conjugates 17–21 ) and 1 μM, 10 μM and 100 μM (conjugates 27–31 ) or the corresponding vehicle (0.1% DMSO).

Techniques: Concentration Assay, Activity Assay, Incubation, Positive Control, Control